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. 2021 Nov 15;13(22):5703. doi: 10.3390/cancers13225703

Figure 4.

Figure 4

Reduction of CTNND1 upregulates the CXCR4/CXCL12 axis to facilitate TNBC cells homing to the bone. (A) Expression of bone metastasis-related genes was detected by qRT-PCR in human TNBC cell lines (MDA-MB-231, MBA-MB-468) with and without CTNND1 knockdown. The result was showed as a heatmap. (B) Cytokines secreted by MDA-MB-231 with CTNND1 knockdown were detected by human Cytokine Array Kit. (C) Immunoblotting of CXCR4 in TNBC cells with CTNND1 knockdown. (D) Immunofluorescence of CXCR4 in TNBC cells with CTNND1 knockdown. Scale bar = 50 μm. (E) Images and quantification of chemotaxis of TNBC cells with CTNND1 knockdown, fibroblast (upper panel), osteoblast (middle panel), CXCL12 (lower panel) in bottom chamber, respectively. Scale bar = 200 μm. *** p < 0.005. Error bars indicate Standard Error of Mean (S.E.M.).