Skip to main content
. 2021 Nov 15;13(22):5703. doi: 10.3390/cancers13225703

Figure A2.

Figure A2

(A) Cartoon exemplifying the silent mutations in the siRNA1 and siRNA2 target sequence of WT CTNND1 cDNA, which confer resistance to the siRNA1 and siRNA2 and no change in the amino-acid sequence of the resulting protein (mut-CTNND1), compared with the WT. (B) Images and quantification of chemotaxis of MDA-MB-231 and MDA-MB-468 cells expressing mut-CTNND1 despite co-transfection with siRNA1 and siRNA2. (C) Immunoblot of CTNND1 and CXCR4 in MDA-MB-231 and MDA-MB-468. Note the strong expression of CTNND1 in cells expressing mut-CTNND1 despite co-transfection with siRNA1 and siRNA2. (D) Expression of CTNND1 and CXCR4 by qRT-PCR in MDA-MB-231 and MDA-MB-468 transfected with the other two sh-CTNND1 plasmids. (E) Images and quantification of transwell migrated and invaded cells after CTNND1 knockdown by being transfected with the other two sh-CTNND1 plasmids. Scale bar represents 200 μm. All experiments were repeated at least 3 times. * p < 0.05, ** p < 0.01, *** p < 0.005. Error bars indicate Standard Error of Mean (S.E.M.).