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Comparison of the pluripotency of hPSCs in 2D/monolayer and 3D/EB culture systems. (A) Experimental procedure of in vitro differentiation in parallel settings showing representative phase-contrast images of cells at Day 0, Day 4, Day 6 and Day 8. (B) Immunofluorescence analysis of 3D/EBs clones and their cross-sections, and 2D/Monolayer cells, stained by antibodies to Tra-160, Oct4 and NANOG. Scale Bars: EB: 100 μM, Monolayer: 100 μM.
