Table 1.
Selected publications on the independent use of 3D/Embryoid Body and 2D/monolayer systems in hematopoietic differentiation from hPSCs. The table displays the differences in the generation of hematopoietic lineages under these two conditions. Additionally, results show the importance of Wnt/β-catenin signaling activation during in vitro differentiation of hPSCs of the definitive hematopoietic fate.
| Study | hPSCs | Culture System | Day(s) Hematopoietic Commitment | Hematopoietic Wave | Lineage Cells | Wnt Activation |
|---|---|---|---|---|---|---|
| Yanagimachi et al., 2013 | hESCs, hiPCSs | 2D/Monolayer | 6 | Primitive | Monocytes, Dendritic Cells | No |
| Sturgeon et al., 2014 | hESCs, HiPSCs |
3D/EB | 6 | Primitive definitive |
Monocytes, Lymphoid Lineages | Yes |
| Ruiz et al., 2019 | hiPCSs | 2D/Monolayer | 5–9 | Primitive | Primitive Hematopoietic Cells | Yes |
| Niwa et al., 2011 | hiPSCs | 2D/Monolayer | 6 | Primitive | Monocytic Lineages | No |
| Kennedy et al., 2007 | hESCs | 3D/EB | 5–6 | Primitive | Early Hematopoietic Progenitors | No |
| Galat et al., 2017 | hiPSCs | 2D/Monolayer | 5 | Primitive | Monocytic lineages | Yes |
| Ditadi & Sturgeon, 2015 | hESCs | 3D/EB | 8–15 | Primitive, Definitive progenitors | Hemogenic Endothelium and early hematopoietic progenitors | Yes |
| Knorr et al., 2013 | hIPSC, hESCs | 3D/EB | 11 | Definitive | Nk, Lymhpoid | No |