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. 2021 Oct 21;10(11):2822. doi: 10.3390/cells10112822

Figure 2.

Figure 2

iPSC-derived monocyte/macrophage cells display a decreased cell growth rate. (A) Number of the colonies of CD45+ cells seeded in semi-solid culture condition represented as mean with SEM (n = 3 independent experiments). Cells were seeded and replated every 6 days for three times (p1, p2, p3). CBE 250 µM was added to CTRL cells at every replating. (B) Count of the iPSC-derived monocyte/macrophages growth rate seeded in liquid culture with VEGF 50 ng/µL, bFGF 50 ng/µL, SCF 50 ng/µL, Flt3L 5 ng/µL, IL3 25 ng/µL, M-CSF 50 ng/µL, GM-CSF 25 ng/µL. For CTRL + CBE condition, CBE 250 µM was added to the medium at every cell passage. Graphs represent mean with SEM (n = 3). (C) Measurement of cell proliferation with the WST1 reagent of CTRL and CTRL treated with CBE iPSC-derived monocyte/macrophages after 19 days of differentiation. Histograms represents mean with SEM. Statistical significance is indicated (Student’s t-test), * p < 0.05, ** p < 0.01.