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. 2021 Nov 10;13(22):5620. doi: 10.3390/cancers13225620

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In-house cohort validation (A–C) Western blot detection of GSDMD and GSDME expression in glioma tissues and nontumor brain tissues (NBT). GAPDH was used as internal reference. (D–F) IHC staining of GSDMD in NBT, lower-grade glioma (LGG) and glioblastoma (GBM). (G–I) IHC staining of GSDMD in NBT, LGG and GBM. (J) Kaplan–Meier analysis was employed to assess the association between GSDMD/GSDME expression and the prognosis of glioma patients. ** p < 0.01. ns, no significance. HR, hazard ratio.