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. 2021 Nov 10;13(22):5620. doi: 10.3390/cancers13225620

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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GSDMD mediated TMZ-induced pyroptosis in glioma. (A) Morphological changes in U251 cells treated with different concentrations of TMZ. Cells presented with large bubbles emerging from the plasma membrane (red arrows). (B,C) ELISA kits were used to measure the level of LDH and IL-1β after treatment with TMZ. * p < 0.05. ** p < 0.01. *** p < 0.001. ns, no significance. (D) Expression of GSDMD, cleaved caspase 1 and IL-1β were detected using WB in U251 and U87 cells treated with TMZ. GAPDH was used as internal reference. (E,F) After transfection of siRNA against GSDMD, the expression of GSDMD and IL-1β were examined by WB. Morphological changes were observed (red arrows). (G,H) Prognostic role of GSDMD in predicting TMZ response in glioma patients in CGGA. HR, hazard ratio.