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. 2021 Nov 16;13(22):5738. doi: 10.3390/cancers13225738

Figure 1.

Figure 1

Generation of CARs and analysis of IL-2 secretion by c-Met CAR Jurkat cell. (A) Schematic representation of c-Met CAR and FITC CAR construct. (B) c-Met gene (pCMV3-Met) was transduced by electroporation into K562, and Western blot was performed. (C) After transducing the mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR construct into Jurkat, expressions of CAR were confirmed through Western blot. (D) The mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR Jurkat were co-cultured with K562 or c-Met-K562 at an E:T ratio of 15:1. After overnight incubation, supernatants were collected and ELISA was performed to measure IL-2 level. (The whole western blots figure see Figure S1).