Figure 3.

c-Met CAR Jurkat specifically recognizes the c-Met positive GC cells. (A) The mock CD8sp-c-Met CAR, or CSF2Rsp-c-Met CAR Jurkat cells (1.5 × 10⁵ cells) were co-cultured with GC cell lines (1 × 10⁴ cells) at an E:T ratio of 15:1. After overnight incubation, supernatants were collected and ELISA was performed to measure IL-2 level. The bars show mean ± S.D. of experiments performed in triplicate. Statistical analysis was performed by the paired t-test. *** p ≤ 0.01, compared with the mock Jurkat group. (B) The mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR Jurkat cells were co-cultured with GC cell lines in the presence or absence of purified soluble c-MET protein. The next day, supernatants were collected and IL-2 ELISA was performed. The bars show mean ± S.D, of experiments performed in triplicate. Statistical analysis was performed by the paired t-test. *** p ≤ 0.01, compared with the c-Met absence group.