Figure 4.

c-Met CAR KHYG-1 specifically lyses the c-Met positive GC cells. (A) Western blot with the cell lysates of mock CD8sp-c-Met, CSF2Rsp-c-Met, or FITC KHYG-1 cells to see the CAR expression (B) The mock CD8sp-c-Met CAR, CSF2Rsp-c-Met CAR, or FITC CAR KHYG-1 were co-incubated with MKN-45, SNU-5, SNU-1, and SNU-484 at E:T ratio of 5:1 or 10:1 for 5 h. The cytotoxicity of CAR KHYG-1 was measured by the Bright-Glo (luciferase) assay system. (The whole western blots figure see Figure S1).