Figure 1.

Gfi1 expression in the mouse pancreas. The expression levels of Gfi1 were assessed by RT-qPCR using RNAs from whole pancreata from control mice (n = 6) at different developmental and adult (st) ages (* p < 0.05, ** p < 0.01). (A). Antibodies specifically recognizing Gfi1 are not commercially available. The Gfi1 expression pattern was therefore determined using a transgenic mouse line expressing a reporter gene under the control of Gfi1 promoter (B). Specifically, Gfi1-Cre animals (the Cre recombinase being knocked-in into the Gfi1 locus) were crossed with the Rosa26-β-gal mice (harboring a transgene composed of the ubiquitous Rosa26 promoter upstream of a LoxP-STOP-LoxP cassette followed by the β-galactosidase cDNA). An X-gal staining was performed on 4-month-old Gfi1Cre::RosaLac animals (the blue channel was converted in green to increase the image readability) to reveal β-galactosidase activity. Objective magnification: 20×. Scale bar: 50 μm (C–E).