Figure 5.

Gfi1 is crucially involved in the regulation of Nkx6.2 expression in mouse pancreas. Expression levels of Nkx6.2 measured by RT-qPCR during the embryonic development and adulthood in the pancreas of Pdx1Cre::Gfi1cko animals and age-matched controls (n = 5 animals per genotype per (st) age). Insert graph shows Nkx6.2 expression exclusively in control pancreatic samples. (* p < 0.05, ** p < 0.01) (A). Nkx6.2 expression pattern was assessed by RNA scope in adult Gfi1 loss of function and control pancreatic sections. Yellow dash line indicates the islet compartment. Objective magnification: 25×. Scale bar: 50 μm (B,C).