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. 2021 Oct 20;10(11):2805. doi: 10.3390/cells10112805

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Pdx1Cre::Gfi1cko mice are protected against chemically-induced diabetes. The expression levels of goat were assessed by RT-qPCR in Gfi1 mutant and control pancreatic samples during embryonic development and postnatally (n = 5 animals per genotype per (st) age) (* p < 0.05, ** p < 0.01) (A). ELISA immunodetection of acyl- and deacyl-ghrelin was performed on protein lysates from whole pancreatic tissue extracted from control and Gfi1 mutant mice. (n = 6 animals per genotype) (** p < 0.01, **** p < 0.0001) (B). Immunohistochemical evaluation of pancreatic islets of Langerhans targeting active caspase-3 (in red) of control and Gfi1 mutant mice 24 h after treatment with or without high doses of STZ. Objective magnification: 20×. Scale bar: 50 μm (C–E). Immunohistochemical assessment of β-cell mass was performed on sections isolated from control and Pdx1Cre::Gficko mice following 7 days of STZ treatment (F,H) and non-treated controls (G). Objective magnification: 10×. Scale bar: 500 μm. Basal glycemia of STZ-treated control and Gfi1-deficient mice for 7 weeks. (n = 8 animals per genotype) (** p < 0.01, *** p < 0.001, **** p < 0.0001) (I).

Pdx1Cre::Gfi1cko mice are protected against chemically-induced diabetes. The expression levels of goat were assessed by RT-qPCR in Gfi1 mutant and control pancreatic samples during embryonic development and postnatally (n = 5 animals per genotype per (st) age) (* p < 0.05, ** p < 0.01) (A). ELISA immunodetection of acyl- and deacyl-ghrelin was performed on protein lysates from whole pancreatic tissue extracted from control and Gfi1 mutant mice. (n = 6 animals per genotype) (** p < 0.01, **** p < 0.0001) (B). Immunohistochemical evaluation of pancreatic islets of Langerhans targeting active caspase-3 (in red) of control and Gfi1 mutant mice 24 h after treatment with or without high doses of STZ. Objective magnification: 20×. Scale bar: 50 μm (C–E). Immunohistochemical assessment of β-cell mass was performed on sections isolated from control and Pdx1Cre::Gficko mice following 7 days of STZ treatment (F,H) and non-treated controls (G). Objective magnification: 10×. Scale bar: 500 μm. Basal glycemia of STZ-treated control and Gfi1-deficient mice for 7 weeks. (n = 8 animals per genotype) (** p < 0.01, *** p < 0.001, **** p < 0.0001) (I).