Table 1.
Key reagents and resources.
| Reagent or Resources | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Guinea pig polyclonal anti-insulin | Dako, Hovedstaden, Denmark | Ref: A0564 |
| Rat monoclonal anti-BrdU | Abcam, Cambridge, UK | Ref: ab6326 |
| Rabbit polyclonal anti-pancreatic amylase | ThermoFisher, Waltham, MA, USA | Ref: PA5-25330 |
| Mouse monoclonal anti-ghrelin | Merck, Readington Township, NJ, USA | Ref: MAB10404 |
| Rabbit polyclonal active caspase-3 | R&D Systems, Minneapolis, MN, USA | Ref: AF835 |
| Rabbit monoclonal anti-proglucagon | Cell Signaling, Danvers, MA, USA | Ref: 8233 |
| Rabbit polyclonal anti-somatostatin | Dako, Hovedstaden, Denmark | Ref: A0566 |
| Rabbit polyclonal anti-pancreatic polypeptide | Millipore, Burlington, MA, USA | Ref: AB939 |
| Goat anti-guinea pig IgG (H+L) Alexa Fluor 488 conjugated | ThermoFisher, Waltham, MA, USA | Ref: A-11073 |
| Goat anti-guinea pig IgG (H+L) Alexa Fluor 594 conjugated | ThermoFisher, Waltham, MA, USA | Ref: A-11076 |
| Goat anti-rat IgG (H+L) Alexa Fluor 594 conjugated | ThermoFisher, Waltham, MA, USA | Ref: A-11007 |
| Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 conjugated | ThermoFisher, Waltham, MA, USA | Ref: A-21206 |
| Goat anti-Mouse IgG (H+L) Alexa Fluor 594 conjugated | ThermoFisher, Waltham, MA, USA | Ref: A-11005 |
| Donkey anti-Rabbit IgG (H+L) Alexa Fluor 594 conjugated | ThermoFisher, Waltham, MA, USA | Ref: A-21207 |
| Experimental Models: Organisms/Strains | ||
| Pdx1-Cre | [21] | N/A |
| Gfi1cko | [22] | N/A |
| Gfi1-Cre | [19] | N/A |
| ROSA26-lox-Stop-lox-β-Gal | [20] | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| d-(+)-glucose | Sigma-Aldrich, St. Louis, MO, USA | Ref: G7528-1KG |
| Insulin | Novo Nordisk, Bagsvaerd Denmark | N/A |
| Streptozocin | Sigma-Aldrich, St. Louis, MO, USA | Ref: S0130 |
| RNAlater | Invitrogen, Waltham, MA, USA | Ref: AM7021 |
| Collagenase | Sigma-Aldrich, St. Louis, MO, USA | Ref: C7657 |
| Histopaque-1077 | Sigma-Aldrich, St. Louis, MO, USA | Ref: 10771 |
| Histopaque-1119 | Sigma-Aldrich, St. Louis, MO, USA | Ref: 11191 |
| Antigenfix | Microm Microtech, Brignais, France | Ref: F/P0016 |
| Antigen Unmasking Solution | CliniSciences, Nanterre, France | Ref: H-3300 |
| Tissue freezing medium | Leica, Wetzlar, Germany | Ref: 14020108926 |
| Amylase from porcine pancreas | Sigma-Aldrich, St. Louis, MO, USA | Ref: A6255 |
| Critical Commercial Assays | ||
| RNeasy Mini Kit | Qiagen, Hilden, Grmany | Ref: 74104 |
| Unacylated Ghrelin (mouse, rat) Express Enzyme Immunoassay kit | Bertin Bioreagent, Frankfurt am Main Germany | Ref: A05118 |
| Acylated Ghrelin (mouse, rat) Express Enzyme Immunoassay kit | Bertin Bioreagent, Frankfurt am Main Germany | Ref: A05117 |
| Pierce™ BCA Protein Assay Kit | ThermoFisher, Waltham, MA, USA | Ref: 23225 |
| RNAscope 2.5 HD Reagent Kit-BROWN | Advanced Cell Diagnostics, Newark, CA, USA | Ref: 322370 |
| Software and Algorithms | ||
| Prism software (Graphpad) | GraphPad Software, Inc. 7 | N/A |
| FIJI | imageJ-win64 | N/A |
| ZEISS Axiovision | Carl Zeiss AG Axiovision Rel. 4.8 | N/A |
| Others | ||
| ONETOUCH Vita glucometer | Life Scan, Milpitas, CA, USA | N/A |