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. 2021 Nov 3;10(11):2992. doi: 10.3390/cells10112992

Figure 6.

Figure 6

Induction of VPA and rapamycin on FLT3-ITD the downstream signaling and cell viability in Ba/F3 G613E and MOLM13 cells. Ba/F3 cells that were stably expressing FLT3-ITD G613E variant or MOLM13 cells were treated with 3 mM VPA alone, either 30 nM (Ba/F3) or 5 nM (MOLM13) rapamycin or a combination of both compounds as indicated. (A) The cell lysates were subjected to SDS-PAGE to analyze expression and phosphorylation status of signaling proteins. For the loading control, the levels of GAPDH were detected. The representative blots of two independent experiments are shown. (B) MOLM13 cells were incubated with VPA and/or rapamycin as indicated prior to viability analysis by PrestoBlue assay. (C) Ba/F3 cells that were either expressing the 598/599 or the G613E variant were incubated with VPA and/or rapamycin as indicated with or without 1 mM ATRA. the cell viability analyses were performed by PrestoBlue assay. The graphs are shown as mean ± SD from three independent experiments. Student’s t-test was used for calculating the statistical significance (**** p < 0.0001, *** p < 0.001, ns not significant).