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. 2021 Nov 15;13(22):5710. doi: 10.3390/cancers13225710

Figure 3.

Figure 3

Cystic fibrosis transmembrane conductance regulator (CFTR) and cingulin (CGN) are direct targets of miR-125b. (A) Heatmap showing the 46 downregulated genes in Caco2 cells after transfection of miR-125b. The scale bar shows color-coded differential expression from the mean. (B) Bioinformatics analysis by TargetScan identified one binding site of miR-125b in CFTR 3′ untranslated region (3′ UTR) and two binding sites in CGN 3′ UTR. (C) Relative luciferase activity in SW480 cells cotransfected with wild-type or mutated reporter plasmids and miR-125b or miR-ctrl. (D,E) qPCR analysis of CFTR and CGN mRNA levels in the indicated CRC cells. (F) Western blotting analysis of CFTR and CGN protein levels in the indicated CRC cells. β-actin was used as a loading control. (G) Correlation analysis between miR-125b expression and CFTR or CGN mRNA expression in 20 cases of CRC. * p < 0.05, ** p < 0.01 by the Student’s t-test in (C–E), and by Pearson’s correlation analysis in (G).