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. 2021 Nov 15;13(22):5710. doi: 10.3390/cancers13225710

Figure 6.

Figure 6

miR-125b induces epithelial-mesenchymal transition (EMT) and urokinase plasminogen activator (uPA) secretion by targeting CFTR. (A,B) Transwell assays of Caco2 (A) and SW480 (B) cells cotransfected with miR-125b or miR-ctrl and the CFTR plasmid or control vector. (C) Bioluminescent images and radiance values of mice (n = 8) injected with SW480 cells after the indicated transduction. (D) Representative H&E staining images of lung tissue sections from the indicated groups. Scale bars represent 500 μm. (E) CFTR plasmid containing either with or without 3′ UTR was transfected into HCT15 cells, and western blotting was performed 48 h after transfection (upper). HCT15 cells were transfected with CFTR plasmid containing wild-type 3′ UTR or lacking 3′ UTR along with miR-125b or vector control (lower). (F) Migration and invasion assays of HCT15 cells transfected with the CFTR expression plasmid with or without 3′ UTR along with miR-125b or miR-ctrl. (G) Western blotting analysis of uPA in the indicated cells. (H) Western blotting analysis of EMT markers in the indicated cells. * p < 0.05, ** p < 0.01 by one-way ANOVA followed by Dunnett’s multiple comparison.