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. 2021 Nov 15;13(22):5710. doi: 10.3390/cancers13225710

Figure 7.

Figure 7

miR-125b activates Ras Homolog Family Member A (RhoA)/Rho Kinase (ROCK) signaling by targeting CGN. (A,B) Transwell assays of Caco2 (A) and SW480 (B) cells transfected with miR-125b or miR-ctrl and the CGN plasmid or control vector. (C) Bioluminescent images and radiance values of mice (n = 8) injected with SW480 cells after the indicated transduction. (D) Representative H&E staining images of lung tissue sections from the indicated groups. Scale bars represent 500 μm. (E) CGN plasmid containing either with or without 3′ UTR was transfected into HCT116 cells, and western blotting was performed 48 h after transfection (upper). HCT116 cells were transfected with CGN plasmid containing wild-type 3′ UTR or lacking 3′ UTR along with miR-125b or vector control (lower). (F) Migration and invasion assays of HCT116 cells transfected with the CGN expression plasmid with or without 3′ UTR along with miR-125b or miR-ctrl. (G,H) Western blotting analysis of proteins in RhoA/ROCK pathway in SW480 (G) and Caco2 (H) cells after indicated transduction. * p < 0.05, ** p < 0.01 by one-way ANOVA followed by Dunnett’s multiple comparison.