Figure 2.

Conditioned medium from lung-resident MSCs (LMSCs) alters recovery of the A549 epithelial cell monolayer after wounding by electroporation. A549 cells were seeded in duplicates in ECIS arrays and grown to confluence for 48 h. Medium was replaced by control medium (negative control), medium containing 1% FCS (positive control) or conditioned medium (CM) from LMSCs of non-COPD control and COPD donors. After 24 h, cells were wounded by electroporation. (A) Resistance was measured at a frequency of 400 Hz; a representative plot is shown. (B) Capacitance was measured at a frequency of 40 kHz, a representative plot is shown. (C) Resistance levels were normalized to the values immediately after wounding in the absence and presence of LMSC-CM from a non-COPD or COPD donor in 4 independent experiments. Mean ± SEM levels are shown (n = 8, non-COPD and COPD LMSC-CM combined). (D) Resistance levels were normalized to the values immediately after wounding in the presence of LMSC-CM from non-COPD and COPD donors. Mean ± SEM levels are shown (n = 4/group). To test for differences between control medium and LMSC-condition medium, 2-way ANOVA was used. * = p <0.05 between the indicated values measured over the last 6 h.