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. 2021 Nov 12;13(22):5667. doi: 10.3390/cancers13225667

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CBD inhibits angiogenic potential in breast cancer cell lines. (A) MCF7 and MDA-MB-231 cells were treated with CBD (2 μM) in hypoxia. The total mRNA level of VEGF was analyzed by performing qRT-PCR. (B) The amount of secreted VEGF protein in MCF7 and MDA-MB-231 cells was analyzed by ELISA Kit. ***, p < 0.001 (C) The angiogenic potential was determined by HUVEC tube-formation assay. Conditioned media was collected from the breast cancer cell lines in hypoxia. Early-passage HUVECs were grown on matrigel-coated 48-well plates with conditioned media for 6 h. Capillary tube formation in each well was captured using a light microscope. (D) Total tube length was quantified using the ImageJ program. *, **, and *** indicate p < 0.05, p < 0.01, and p < 0.001 CBD; cannabidiol, CN; control.