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. 2021 Nov 5;10(11):3029. doi: 10.3390/cells10113029

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mitochondrial function assessment by high-resolution respirometry in all different experimental groups. Respiratory states 3 (ADP-stimulated), 4 (non-phosphorylating), and uncoupled (U, CCCP-stimulated) were measured by oxygen consumption rates (pmol/s/mg/protein) in mitochondrial preparations from the left kidney (A) and heart (B) of mice. (A) Cardiac mitochondria oxygen consumption on states 3, 4, and U (pmol/s/mg/protein) (B). Data are expressed as the mean ± standard error of mean (SEM). One-way ANOVA followed by the Tukey’s post hoc test were performed for the selected pairs and number of animals within the bars. a vs. sham, p < 0.05. b vs. IR8d, p < 0.05. c vs. IR15d, p < 0.05.