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. 2021 Nov 7;10(11):3065. doi: 10.3390/cells10113065

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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qRT-PCR validation of the glycolytic genes in various cell lines. (a) U87MG, (b) DBTRG, (c) LN18, (d) LN229, and (e) U118MG. The relative expression of the genes (compared to ACTB, encoding actin) was analyzed in control and NVP-BEZ235 (NVP) samples (1 μM treatment for 5 days). The error bars represent the standard deviation of the genes (n = 4). Asterisks (*) represent the significantly expressed genes (p < 0.05) as compared to control (DMSO). Significance was determined by Student’s t-test.