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. 2021 Nov 7;10(11):3065. doi: 10.3390/cells10113065

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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qRT-PCR validation of the glycolysis-related genes under serum starvation in (a) U87MG, and (b) LN18 cell lines grown in serum-free medium. The relative expression of the genes (compared to ACTB, encoding actin) was analyzed in control DMSO and NVP-BEZ235 samples (five days 1 μM NVP-BEZ235 with no FBS). The error bars represent the standard deviation of the genes (n = 4). Asterisks (*) represent the significantly expressed genes (p < 0.05) as compared to control (DMSO). Significance was determined by Student’s t-Test.