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FOXO1 inhibition partially suppressed NVP-BEZ235 induction of glycolytic genes. (a) U87MG and (b) DBTRG. The relative expression of the genes was analyzed in DD-, DN-, DF-, and NF-treated samples. DD: DMSO; DN: DMSO+NVP-BEZ235 (1 μM); DF: DMSO+FOXOi (1 μM AS1842856); NF: NVP-BEZ235+FOXOi (1 μM of each drug). The error bars represent the standard deviation of the genes. The p-values were calculated by one-way ANOVA followed by Tukey’s test (*, p < 0.05; **, p < 0.01, ***, p < 0.001).
