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. 2021 Oct 20;10(11):2812. doi: 10.3390/cells10112812

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Knockdown of IgM enhanced the migratory ability of murine macrophages through the Src/FAK axis. IgM expression was determined by RT-PCR (A) and Western blot (B) in the RAW264.7 cells by specific siRNA against IgM (si-Igμ) and control siRNA (siNC). (C) The effects of IgM siRNA on cell migration were analyzed by transwell assay, showing that IgM knockdown promoted the migration ability of RAW264.7 cells. Scale bar = 200μm. (D) Data are expressed as the mean ± SD of migrated cells number per five fields. (E) The effects of IgM siRNA on cell proliferation were analyzed by CCK8 assay. The data are presented as mean ± SD (n = 3). (F) Western blot analysis of Src/FAK signaling after IgM knockdown for 24 h. β-actin as an internal control. ** p < 0.01, **** p < 0.0001 vs. control siRNA.