Figure 6.

IgM knockdown promoted the IRE1α branch of the ER stress pathway through regulating Bip expression. RAW264.7 cells were transfected with IgM siRNA (si-Igμ) and control siRNA (siNC) for 36 h before exposure to LPS (100 g/mL) for the indicated times. (A) Intracellular Bip protein expression was upregulated after IgM knockdown by Western blot analysis. β-actin acted as an internal control. (B) Enhanced effects of IgM knockdown on phosphorylation of IRE1α but not PERK, ATF6, and CHOP were shown by Western blot analysis. (C) RT-PCR analysis showing the Expression of XBP1u mRNA and XBP1s mRNA. IgM as positive control and β-actin as an internal control. (D) Representative flow cytometric histograms showing ROS production measured by the H2DCF-DA probe in RAW264.7 cells. blue line, si-Igμ1; red line, si-Igμ2; black line, siNC as control siRNA.