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. 2021 Oct 20;10(11):2813. doi: 10.3390/cells10112813

Figure 9.

Figure 9

Minigene splicing assays in HEK293T cells: Effect of luteolin and kinetin. (a) AGA knockout HEK293T cells were transfected with the minigene-luciferase construct and treated with increasing amounts of substances for 24 h. Relative luciferase activity of the untreated samples was set as 1. Bars represent the mean ± SD of at least three independent experiments, one-way ANOVA against the untreated samples. (b) Protein lysates from (a) were analysed by SDS-PAGE and Western blot. Luciferase expression corresponds to the luciferase activity shown in (a). (c) AGA knockout HEK293T cells were transfected with the AGA minigene construct without luciferase and treated with 25 µM luteolin or 50 µM kinetin for 24 h. Total RNA was isolated, reversely transcribed and analyzed by qRT-PCR. Bars represent the mean ± SD of six independent experiments, one-way ANOVA against the untreated samples. Values of p < 0.01 were considered very significant (**) and p < 0.001 extremely significant (***).