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. 2021 Oct 20;10(11):2813. doi: 10.3390/cells10112813

Figure 10.

Figure 10

Luteolin treatment increases AGA mRNA amount and AGA activity in c.128-2A>G AGU patient fibroblasts. (a) Quantitative real-time PCR of AGA mRNA with patient fibroblasts with c.128-2A>G variant after treatment with luteolin (25 µM) or kinetin (50 µM) for 24 h. AGA was detected with the primers specific for exons 2 and 3. Bars represent the mean ± SD of three independent experiments, one-way ANOVA against the untreated sample. (b) Treatment of fibroblasts with luteolin (25 µM) for 48 h and measurement of AGA activity with AADG as substrate, 13 independent experiments, two-way ANOVA against the untreated sample. (c) Control and patient fibroblasts were treated with suboptimal amounts of caffeine (2.5 mM) to prevent mRNA degradation, either alone or in combination with luteolin (25 µM) for 48 h. AGA activity was measured as in (b), three independent experiments, one-way ANOVA against the untreated sample. Values of p < 0.05 were considered significant (*) while values of p < 0.01 were considered very significant (**).