Figure 11.

TPP1 minigene luciferase splicing assay in HEK293T cells: Effect of methylxanthines, luteolin, and kinetin. (a) Schematic drawing of the TPP1 minigene construct containing the variant c.509-1G<C, with C-terminal firefly luciferase. (b) HEK293T cells were transfected with the TPP1 luciferase minigene construct containing the variant c.509-1G<C and treated with the indicated substances for 24 h. Relative luciferase activity of the untreated samples was set as 1. Bars represent the mean ± SD of at least five independent experiments, one-way ANOVA against the untreated samples. Values of p < 0.01 were considered very significant (**) and p < 0.001 extremely significant (***). (c) Protein lysates from (b) were analyzed by SDS-PAGE and Western blot with an anti-luciferase antibody. Luciferase expression was observed in the samples showing significant luciferase activity in (b).