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. 2021 Oct 20;10(11):2813. doi: 10.3390/cells10112813

Table 1.

Primer sequences for cloning of the AGA and TPP1 minigene constructs. All sequences are shown in 5′ to 3′ direction.

Primer Name Primer Sequence 5′ to 3’
AGA-Minigene fwd HindIII CTATAAAGCTTAGGGACGCCTGAGCGAACCC
AGA-Minigene rev XhoI CTATACTCGAGCTGACTCTCCTACTAAAAGTGTGT
TPP-IVS-Minigene fwd BamHI CTAGGATCCATGCAAGCAGAGCTGCTGCTCCCTG
TPP-IVS-Minigene rev XhoI CTATACTCGAGCAGGGCTACTGTAGACCCAGGTG
Luciferase fwd XhoI CTATACTCGAGAAGACGCCAAAAACATAAAGAAAGG
Luciferase rev XbaI CTATATCTAGATTACACGGCGATCTTTCCGCC