Figure 3.

Simvastatin favors the formation of DNA adducts with cisplatin and ifosfamide. (A–C) Analysis of PCR products by agarose gel electrophoresis. Cells were exposed to increasing concentrations of cisplatin (A) or ifosfamide (C) for 1 h in the presence of simvastatin (10 µM) or solvent. Genomic DNA was extracted and subjected to a PCR stop assay. (B–D) PCR amplification efficacy according to the indicated drug concentration for solvent- or simvastatin-treated cells. Results are expressed as mean ± SD (n = 3) of relative quantification of a signal after normalization for the nested fragment. a: p < 0.05 vs. untreated, b: p < 0.05 vs. solvent.