Figure 4.

Simvastatin favors doxorubicin accumulation into osteosarcoma cells nuclei. (A) Intracellular uptake of doxorubicin. Cells were exposed to doxorubicin (3.6 µM) for 30 min in the presence or absence of simvastatin (10 µM). The image was taken by fluorescent microscope equipped with a digital camera. Enlarged images are shown in the inserts. (B) Distribution of red fluorescent signal intensity as a function of the frequency of occurrence for vehicle-treated cells (light gray) and simvastatin-treated cells (dark gray). Results are also expressed as a box plot. Lower, middle, and top lines of boxes indicate lower quartile, median, and upper quartile, respectively. Whiskers indicate minima and maxima.