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. 2021 Nov 22;13(22):5869. doi: 10.3390/cancers13225869

Figure 7.

Figure 7

Chemotherapy drugs and simvastatin synergistically reduce osteosarcoma cell invasiveness in vitro. (A) Cell motility evaluated by wound-healing assay. Cells were exposed to the single or combined indicated compounds in the presence of the broad caspases inhibitor zVAD-fmk (20 µM). Pictures were taken at times 0 and 18 h after the wound. (B) Relative migrating cell number in wound-healing assay. Results are expressed as mean ± SD (n = 3–4) of two independent experiments. (C) Cell invasion evaluated by Matrigel-coated Boyden chambers. Cells were exposed to the indicated single or combined compounds for 16 h. (D) Relative invading cell number. Results are expressed as mean ± SD (n = 3) of two independent experiments. (E) Matrix metalloproteinase 2 (MMP2) activity evaluated by a colorimetric assay. Cells were exposed to the single or combined indicated compounds for 24 h. Results are expressed as mean ± SD (n = 5–6) of two independent experiments. a: p < 0.05 compared to untreated, b: p < 0.05 compared to simvastatin alone, c: p < 0.05 compared to chemotherapy drug alone.