Figure S2. Pre-hearing Manf_fl/fl;Pax2-Cre B6 mice show normal morphology of the outer hair cell (OHC) hair bundles in the low-frequency region of the cochlea.

(A, B) At P9, SEM analysis in the 16-kHz cochlear region shows a comparable complement of OHC hair bundles in cKO and control mice. (C, D) High-magnification images of single bundles show no apparent differences in stereocilia length or organization. (E, F) High-magnification images of 16-kHz OHC hair bundles show inter-stereociliary links (arrowheads) in both genotypes. Abbreviations: B6, C57BL/6J strain; cKO, conditional knock out; Ctrl, control; OHC, outer hair cell; SEM, scanning electron microscopy. Scale bars: (A) 5 μm A, B; (C) 1 μm C, D; (E) 200 nm E, F.