Figure S5. Outer hair cells (OHCs) of Manf^fl/fl;Pax2-Cre B6 retain ribbon synapses at adulthood.

(A, B) Double-immunostaining for myosin 7a (blue) and the presynaptic ribbon-marker CtBP2 (white puncta) from the 45-kHz cochlear region at P35 shows that the normal few ribbons per OHC are present in cKO mice. OHCs are arranged in three rows (1,2,3). Note scattered OHC loss (asterisks) in the mutant. Observe also the IHC synaptopathy in the same specimen. Abbreviations: B6, C57BL/6J strain; cKO, conditional knock out; CtBP2, C-terminal Binding Protein 2; Ctrl, control; IHC, inner hair cell; OHC, outer hair cell. Scale bar: (A) 5 μm A, B.