Table 4. Relative binding free energy (kcal/mol) for Q203 in three mutants of QcrB compared to the wild-type (WT).
Mutant | 25–50 ns | 50–75 ns | 75–100 ns | Average | Standard deviation |
---|---|---|---|---|---|
T313A | 6.32 | 11.01 | 8.11 | 8.48 | 2.37 |
T314Y | 4.54 | 6.00 | 8.07 | 6.20 | 1.77 |
T313A + E314Y | 10.28 | 16.30 | 13.64 | 13.41 | 3.02 |