FIG. 1.
Two-hybrid analysis of various RED1 constructs. The two boxes present in Red1p represent regions of predicted coiled-coil structure. Protein-protein interactions were assayed by β-galactosidase filter assays in strain L40. Plasmids used: pNH223 (lexA-RED11–827); pDW35 (lexA-RED11-326); pDW61 (lexA-RED11–359); pDW62 (lexA-RED11-400); pDW28 (lexA-RED11–426); pDW58 (lexA-RED1536–827); pJ63 (GAD-RED11–827); pGAD-RED1537–827 and pDW54 (GAD-RED1330–426). One plus sign indicates that the cells turned blue in 90 min at 30°C; three plus signs indicates the cells turned blue by 15 min; a minus sign indicates a failure to turn blue by 4 h.