TABLE 2.
S. cerevisiae strains used
| Strain | Genotype | Source or reference |
|---|---|---|
| L40 | MATa his3Δ200 trp1-90 leu2-3,112 ade2 lys2::lexA HIS3 LYS2 gal80 | R. Sternglanz |
| ura3::lexA lacZ URA3 | ||
| NH214 | MATa leu2 ura3::pSH18-34ΔSpe (lexAop-lacZ URA3) CAN1 trp1 his3 ade2 red1Δ::ADE2 LYS1 CYH2 tyr1 | This work |
| MATα leu2 ura3::pSH18-34ΔSpe (lexAop-lacZ URA3) can1 trp1 his3 ade2 red1Δ::ADE2 lys1 cyh2 TYR1 | ||
| BR1373-6D | MATa leu2-27 his4-280 ura3-1 ade2-1 thr1-1 trp1-1 spo13::ura3-1 lys2 arg4-8 cyh10 | J. Engebrecht |
| 5787-21-4 | MATα cdc10-2 trp1 ura3 can1 cyh2 spo13-1 ade2-1 | 17 |
| NH246 | MATa CDC10 leu2 his4 arg4-8 thr1-1 ura3-1 CAN1 trp1-1 cyh10 ade2-1 spo13::ura3-1 CYH2 red1Δ::ADE2 | This work |
| MATα cdc10 LEU2 HIS4 ARG4 THR1 ura3 can1 trp1 CYH10 ade2-1 spo13-1 cyh2 red1Δ::ADE2 | ||
| NH246::pRS306 | Same as NH246 except ura3::pRS306 (URA3) ura3::pRS306 (URA3) | This work |
| NH246::pSB3 | Same as NH246 except ura3::pSB3 (RED1 URA3) ura3::pSB3 (RED1 URA3) | This work |
| NH246::pSB3-K348E | Same as NH246 except ura3::pSB3-K348E (red1-K348E URA3) ura3::pSB3-K348E (red1-K348E URA3) | This work |
| NH246::pRS306 Spo13+ | Same as NH246::pRS306 except spo13::pNH20 (TRP1 SPO13) spo13 | This work |
| NH246::pSB3 Spo13+ | Same as NH246::pSB3 except spo13::pNH20 (TRP1 SPO13) spo13 | This work |
| NH246::pSB3-K348E Spo13+ | Same as NH246::pSB3-K348E except spo13::pNH20 (TRP1 SPO13) spo13 | This work |
| RKY1145 | MATa leu2ΔhisG his4-x ura3 lys2 hoΔ::LYS2 | G. S. Roeder |
| S2683 | MATα leu2-K arg4-Nsp ura3 lys2 hoΔ::LYS2 | G. S. Roeder |
| YTS3a | MATa leu2ΔhisG his4-x ARG4 ura3 lys2 hoΔ::LYS2 red1::LEU2 | 11 |
| MATα leu2-K HIS4 arg4-Nsp ura3 lys2 hoΔ::LYS2 red1::LEU2 | ||
| YTS3::pRS306a | Same as YTS3 except ura3::pRS306 (URA3) ura3::pRS306 (URA3) | This work |
| YTS3::pSB3a | Same as YTS3 except ura3::pSB3 (RED1 URA3) ura3::pSB3 (RED1 URA3) | This work |
| YTS3::pSB3-K348Ea | Same as YTS3 except ura3::pSB3-K348E (red1-K348E URA3) ura3::pSB3-K348E (red1-K348E URA3) | This work |
| DW10a | MATa leu2ΔhisG his4-x ARG4 ura3 lys2 hoΔ::LYS2 hop1::LEU2 | 11 |
| MATα leu2-K HIS4 arg4-Nsp ura3 lys2 hoΔ::LYS2 hop1::LEU2 | ||
| NH311::pRS402a | MATa leu2ΔhisG his4-x ARG4 ura3 lys2 hoΔ::LYS2 hop1::LEU2 ade2-Bgl::pRS402 (ADE2) sae2Δ::URA3 | This work |
| MATα leu2-K HIS4 arg4-Nsp ura3 lys2 hoΔ::LYS2 hop1::LEU2 ade2-Bgl::pRS402 (ADE2) sae2Δ::URA3 | ||
| NH212-1-1 | MATa leu2 his4 ura3 lys2 hoΔLYS2 ade2-Bgl red1::LEU2 | This work |
| NH212-35-1 | MATα leu2-k ura3 lys2 hoΔLYS2 arg4-Nsp ade2-Bgl red1::LEU2 | This work |
| NH305b | MATa leu2 his4 ura3 lys2 hoΔLYS2 ARG4 ade2-Bgl red1::LEU2 | This work |
| MATα leu2-k HIS4 ura3 lys2 hoΔLYS2 arg4-Nsp ade2-Bgl red1::LEU2 | ||
| NH305::pRS402b | Same as NH305 except ade2-Bgl::pRS402 (ADE2) ade2-Bgl | This work |
| NH305::pBB16b | Same as NH305 except ade2-Bgl::pBB16 (RED1 ADE2) ade2-Bgl::pBB16 (RED1 ADE2) | This work |
| NH305::pBB14b | Same as NH305 except ade2-Bgl::pBB14 (red1-K348E ADE2) ade2-Bgl::pBB14 (red1-K348E ADE2) | This work |
| NH306b | Same as NH305 except sae2Δ::URA3 sae2Δ-URA3 | This work |
| NH306::pRS402b | Same as NH306 except ade2-Bgl::pRS402 (ADE2) ade2-Bgl::pRS402 (ADE2) | This work |
| NH306::pBB16b | Same as NH306 except ade2-Bgl::pBB16 (RED1 ADE2) ade2-Bgl::pBB16 (RED1 ADE2) | This work |
| NH306::pBB14b | Same as NH306 except ade2-Bgl::pBB14 (red1-K348E ADE2) ade2-Bgl::pBB14 (red1-K348E ADE2) | This work |
| NH306::pBB19 | Same as NH306 except ARG4 arg4::pBB19 (red1-K348E ARG4) | This work |
a
SK1 diploid generated by crossing RYK1145 and S2683 transformed derivatives.
b
SK1 diploid generated by crossing 212-1-1 and 212-35-1 derivatives. 212-1-1 and 212-35-1 are haploid segregants from a cross of transformed derivatives of the SK1 haploids RYK1145 and S2683.