TABLE 5.
Effect on meiotic interhomolog crossing over of red1Δ and red1-K348E mutants
| NH246:: | Relevant genotype | Map distance (centimorgans)a
|
Mean fold reduction | |||
|---|---|---|---|---|---|---|
| HIS4-LEU2 | LEU2-CDC10 | CDC10-MAT | ARG4-THR1 | |||
| pSB3 | RED1 | 18.3 (254) | 13.1 (245) | 22.2 (248) | 12.1 (286) | 1.0 |
| RED1 | ||||||
| pRS306 | red1Δb | 0.7 (284) | 1.6 (286) | 1.7 (295) | 2.0 (318) | 10.9 |
| red1Δ | ||||||
| pSB3-K348E | red1-K348Eb | 4.7 (223) | 7.8 (223) | 9.8 (224) | 6.2 (256) | 2.3 |
| red1-K348E | ||||||
a
Calculated as described by Hollingsworth et al. (21).
b
All values are statistically significantly different from the RED1 values to the P < 0.001 level by χ2 analysis.