Figure 4.

Confocal imaging (A) showed inhibition of CD8⁺ T-cell activation via major histocompatibility complex-I-blocking antibody (MHCi) attenuated total T-cell numbers due to a decrease in the CD8⁺ T-cell population. LPS + MI + MHCi decreased CD44 expression on the CD8⁺ T cells as demonstrated by the lower number of effector (CD3⁺CD8⁺CD44⁺CD27⁻) but not memory (CD3⁺CD8⁺CD44⁺CD27⁺) CD8⁺ T cells compared with the LPS + MI group (B). No differences were observed between the LPS + MI group and the LPS + MI + IgG controls. Overlay images are shown for LPS + MI + MHCi and LPS + MI + IgG groups (for other groups, see Fig. 1). n = 4–12/group; Scale bar: 40 μm; Females are designated by yellow dots and males by blue dots; multiple group comparisons were analyzed by one-way ANOVA with Student–Newman–Keuls posttest; †P < 0.05 vs. MI; ‡P < 0.05 vs. LPS + MI. LPS, lipopolysaccharide; MI, myocardial infarction.