Figure 4.

Histogram showing alkaline phosphatase activity of normal human dental pulp cells in media with supplemental Mg²⁺concentrations. Note: normal human dental pulp cells were cultured with media containing supplemental magnesium concentrations 0.5 mM, 1 mM, 2 mM, 4 mM, and 8 mM and 0 mM as the control group for time periods of 7, 10, and 14 days. Alkaline phosphatase activity in supernatants was normalized per million cells at each time interval. The control cells were treated with growth media without supplemental Mg²⁺. The data are presented as means of six replicates with error bars indicating the standard deviation. Groups labeled with different letters differ statistically as compared to the control group and other study groups (P < 0.0001).