FIGURE 12.

Single chain of the Tuna (T1087) crystal structure (yellow) superimposed with exemplary predictions (red: AlphaFold2; cyan: FEIG‐S, green: BAKER‐ROBETTA). Tuna forms two coiled‐coil interfaces, one intra‐chain and one for homo‐dimerization with another chain. Although only the monomer was a prediction target in CASP 14, several residues of the inter‐chain interface were predicted in the correct rotamer by the top‐ranking groups and servers. The N‐terminal extension containing a polyproline‐II helix is only shown for AlphaFold2, which predicted it most precisely in conformation and position