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. 2021 Nov 26;1(1):29. doi: 10.1186/s44149-021-00026-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — General map of mariner vectors, pTsodCPC9 and pTlacPC9, differing only in the promoter for the tnp gene. Genes tnp (mariner transposase C9 mutant), cat (chloramphenicol resistance), traJ (plasmid transfer gene), and bla (ampicillin resistance) are indicated by the appropriately labelled solid red arrows. The origin of plasmid transfer (oriT) is shown as a filled lollipop (), and the origin of plasmid replication (colE1) as a hollow lollipop (). Coloured blocks flanking the cat gene indicate the locations of Himar1 inverted repeats (thick orange), and paired copies of DNA uptake sequences for Neisseria spp. (thin green), H. influenzae (thin pink) and A. pleuropneumoniae (thin blue). Arrowhead upstream of the C9 tnp gene indicates promoter sequences for either A. pleuropneumoniae sodC or E. coli lac genes, depending on the vector (pTsodCPC9 and pTlacPC9, respectively)

Inline graphic — General map of mariner vectors, pTsodCPC9 and pTlacPC9, differing only in the promoter for the tnp gene. Genes tnp (mariner transposase C9 mutant), cat (chloramphenicol resistance), traJ (plasmid transfer gene), and bla (ampicillin resistance) are indicated by the appropriately labelled solid red arrows. The origin of plasmid transfer (oriT) is shown as a filled lollipop (), and the origin of plasmid replication (colE1) as a hollow lollipop (). Coloured blocks flanking the cat gene indicate the locations of Himar1 inverted repeats (thick orange), and paired copies of DNA uptake sequences for Neisseria spp. (thin green), H. influenzae (thin pink) and A. pleuropneumoniae (thin blue). Arrowhead upstream of the C9 tnp gene indicates promoter sequences for either A. pleuropneumoniae sodC or E. coli lac genes, depending on the vector (pTsodCPC9 and pTlacPC9, respectively)