Table 2.
Location of mariner insertions in anaerobic mutants of P. multocida
| Gene | Product of disrupted gene | Gene locationa | Location of inserta |
|---|---|---|---|
| MIDG3277_1258 | Predicted glycosyl transferase | contig21:104818..105990b | contig21:105340 |
| aroG | Deoxy-7-phosphoheptulonate synthase | contig15:68162..69247 | contig15:68149c |
| intergenic | Periplasmic pH-dependent serine endoprotease DegQ | contig 16:46775..48154 | contig16:46700d |
| MIDG3277_1016 | Hypothetical proteine | contig18:76828..77169C | contig18:77129 |
| aroA | 3-phosphoshikimate 1-carboxyvinyltransferase | contig18:79640..80962 | contig18:80029 |
| contig18:80287 | |||
| sucC | Succinate--CoA ligase subunit beta | contig3:86080..87246 | contig3:86908 |
| ccmD | Heme exporter protein CcmD | contig1:16084..16287 | contig1:16136 |
| MIDG3277_1814 | F0F1 ATP synthase subunit I | contig31:36319..36726 | contig31:36262f |
| atpG | F0F1ATP synthase subunit gamma | contig31:40520..41389 | contig31:40497g |
| MIDG3277_0379 | Hypothetical protein | contig6:168..512h | contig6:434 |
| pfeA | Ferric enterobactin receptor | contig27:62820..64826C | contig27:64243 |
| menA | 1,4-dihydroxy-2-naphthoate octaprenyltransferase | contig23:97896..98801C | contig23:98875 |
| menB | 1,4-dihydroxy-2-naphthoyl-CoA synthase | contig23:34911..35768C | contig23:35383 |
| menC | O-succinylbenzoate synthase | contig23:33645..34658C | contig23:34159 |
aLocation in the draft genome of MIDG3277 (accession number ERZ681052)
bStart and end positions of genes are indicated; those on the complementary strand are shown with the suffix C
cInsertion located 13 bases upstream of the start of aroG; insertion likely disrupts transcription of aroG
dInsertion located 75 bases upstream of the start of degQ and 147 bases upstream of the start of MIDG3277_0890 encoding a putative NAD(P)H nitroreductase; insertion likely disrupts transcription of degQ
eThis protein shares 100% identity with PM0836 (Genbank accession AAK02920) which was reported to be in vivo expressed (Hunt et al. 2001)
fInsertion located 57 bases upstream of the start of atpI (MIDG3277_1814); insertion likely disrupts transcription of atpI and remaining genes in the atp operon
gInsertion located 23 bases upstream of atpG; insertion likely disrupts transcription of atpG and remaining genes in the atp operon
hThis predicted CDS is on a small contig and may be part of a larger gene (100% identity with the last 104 AAs of a 818 AA hypothetical protein P1062_0208970; Genbank accession ESQ71762)