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. 2021 Nov 25;12:6877. doi: 10.1038/s41467-021-27279-4

Fig. 3. Supersized LDs in AGPAT2-depletion cells can be rescued by reducing PA.

Fig. 3

a LD morphology after knocking down DGAT1 or DGAT2 in AGPAT2-deficient cells HeLa cells by siRNA. Cells were treated with 400 μM oleic acid for 18 h. Blue represents DAPI staining, and green represents BODIPY staining. Bars = 5 μm. b Quantification of LD diameters as shown in (a). mean ± SD; ****p < 0.0001; *p < 0.05, two-way ANOVA, n = 50 cells examined over three biologically independent experiments. c LD morphology after overexpressing hCDS1-mCherry, hCDS2-mCherry and mCherry-hSeipin in AGPAT2 knockdown HeLa cells. Cells were treated with 400 μM oleic acid for 18 h. Green represents BODIPY staining and red represents mCherry expression. Bars = 10 μm. d and e Distribution of LDs according to diameters (d) and the number of LDs (e) after the over-expressing mCherry tagged-CDS1, CDS2 and seipin in control and AGPAT2-deficient cells. d LD size was represented by red (>3 µm), green (2–3 µm), white (1–2 µm) and black (0–1 µm). e mean ± SD; ***p < 0.001; **p < 0.01; *p < 0.05, two-way ANOVA, n = 15–30 cells examined over three biologically independent experiments.