Fig. 7. Ablation of AGPAT2 from mouse liver reduces hepatic CDS activity.
a Western blotting of MTP, CDS2 and AGPAT2 from primary hepatocytes isolated from WT (Agpat2f/f, n = 4) and liver-specific AGPAT2 knockout mice (A2LKO/Agpat2f/f, Alb-cre, n = 4). b Total PA levels of WT (n = 4) and A2LKO (n = 4) mice fed chow diet. c Liver TAG and d total cholesterol (TC) of WT (n = 6–9) and A2LKO (n = 5–7) mice fed chow or HFD. e Liver morphology and f the ratio of liver to body weight from WT and A2LKO mice fed HFD. g BODIPY-stained liver sections from WT and A2LKO mice fed HFD. Bars: 50 μm. h Distribution of LDs of different diameters as shown in (g). i CDS activity of WT (n = 4) and A2LKO liver (n = 4). j Primary hepatocytes from WT and A2LKO chow-fed mice were transfected with mCherry-CDS2 and stained with BODIPY. Dashed line indicates cells transfected with mCherry-CDS2. k Distribution of LDs according to diameters as shown in (j). b–d, f and i mean ± SD; two-tailed unpaired t test. *p < 0.05, **p < 0.01, ****p < 0.0001.