Fig. 5. dsRBD supports the 3p-strand cleavage of animal DROSHAs.

a The DROSHA and DGCR8 fragments were purified and used in this figure. The numbers indicate the positions of amino acids at the N- and C-terminus of each protein fragment. The asterisks indicate the mutation sites. Rhed, RNA heme-binding domain; CTT, C-terminal tail. Pri-miRNA cleavage assays. b Five pmol of pri-miRNAs were incubated with 5 pmol of human WT or mutant Microprocessor for 2 h at 37 °C. c, d The cleavage efficiency of the WT and mutant Microprocessor in (b). The cleavage efficiency was estimated as the ratio of the F2 + F3 or F1 + F2 to the original substrates. The p-values of the two-tailed t-test for the relative cleavage efficiency estimated from three replicates were shown. The error bars represent SEM. hMP, human Microprocessor.