Fig. 7. Inducible osteoblastic deletion of Stat3 impaired bone formation and reduced bone mass in adult mice.

a Illustration of inducible Stat3 deletion in Col1a1-expressing osteoblast lineage cells (specifically, the mouse icon is bought from https://www.shutterstock.com). When Col1^{Cre ERT2};Stat3^fl/fl mice were injected with tamoxifen, Cre translocated to the nucleus and exon 18 to 20 domain were deleted (TA: tamoxifen). b Scheme of the experiment: 5-week-old male Stat3^fl/fl and Col1^{Cre ERT2};Stat3^fl/fl mice were injected with tamoxifen (100 mg/kg) for 7 days and euthanized for analysis. c Micro-CT images of the distal femurs from 6-week-old Stat3^fl/fl and Col1^{Cre ERT2};Stat3^fl/fl mice. d–h Quantitative parameters of micro-CT, including BV/TV, Tb.Th., Tb.N., Tb.Sp., and Ct.Th. n = 5. i Images of calcein-alizarin red S double labeling of femurs from 6-week-old Stat3^fl/fl and Col1^{Cre ERT2};Stat3^fl/fl mice and quantitative parameters of MAR. n = 5. Two-tailed Student’s t test. Data represent the mean ± s.d. Source data are provided in the Source data file.