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. 2021 Nov 25;12:6891. doi: 10.1038/s41467-021-27273-w

Fig. 8. Pharmacological inhibition of STAT3 activity inhibited osteoblast differentiation and induced bone loss by impairing osteogenesis.

Fig. 8

a ALP staining and alizarin red S staining of BMSCs after culture in osteogenic medium with or without AG490 for 7 and 14 days separately. b Expression of p-STAT3 and STAT3 in BMSCs with or without AG490 for 12 h analyzed by western blotting. The experiment was performed twice to similar results. c The relative mRNA levels of Runx2, Dlx5, Alp, Col1α1, Ocn, and Bsp in BMSCs with or without AG490 for 14 days were quantified by qPCR. n = 9. d Micro-CT images of distal femurs from 9-week-old vehicle and AG490-injected mice. Four-week-old male mice were injected with or without 5 mg/kg AG490 daily for 5 weeks. e–i Quantitative parameters of micro-CT, including BV/TV, Tb.Th., Tb.N., Tb.Sp., and Ct.Th. n = 5. j Images of calcein-alizarin red S double labeling of femurs from 9-week-old mice with or without AG490 and quantitative parameters of MAR. n = 5. Two-tailed Student’s t test. Data represent the mean ± s.d. Source data are provided in the Source data file.