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. 2021 Nov 25;12:6905. doi: 10.1038/s41467-021-27270-z

Fig. 2. A CRISPR-Cas9-induced t(4;11)/MLL-AF4 translocation in human FL HSPCs causes a dramatic increase in B-lineage cell proliferation in vitro.

Fig. 2

a Cumulative absolute number of human CD45+CD19+ cells per well over time during CRISPRMLL-AF4+ and control MS-5 coculture (n = 3; donors 1–3). ***p < 0.005 (two-way ANOVA with Sidak correction for multiple comparisons). Data are shown as mean ± SEM. Source data are provided as a Source Data file. b RT-qPCR of human CD45+ cells showing expression of MLL-AF4 and AF4-MLL (n = 3; donors 1–3) relative to GAPDH at week 4 of MS-5 coculture. Data are shown as mean ± SEM. Source data are provided as a Source Data file. c. Representative flow cytometry plots of viable, single cells from control and CRISPRMLL-AF4+ cultures on week 4 of coculture. Custom lineage cocktail (Lin) = CD2/CD3/CD14/CD16/CD56/CD235a (Supplementary Table 4). d Quantification of human cell immunophenotypes as a percentage of human CD45+ (hCD45+) cells (left), and progenitor immunophenotypes as a percentage of hCD45+Lin−CD34+ cells (right), in control (n = 3; donors 1–3) and CRISPRMLL-AF4+ (n = 3; donors 1–3) cultures over time. X = data not shown because absolute number of hCD45+ cells is <50. Data are shown as mean ± SEM. Source data are provided as a Source Data file.